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Ampk Alpha1 / PRKAA1

Cat Number: AB-84049
Conjugate: Unconjugated
Size: 100ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human AMPK alpha 1
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 – 1:1000
Immunofluorescence: 1:50 – 1:100

Molecular: 65kDa
Purification: Aff. Pur.
Synonyms: PRKAA1; AMPK; AMPKa1; protein kinase AMP-activated catalytic subunit alpha 1
Background:

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5′-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATPconsuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed

Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-84049-1.jpg

Western blot analysis of extracts of
various cell lines, using PRKAA1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti- Rabbit IgG
(H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

AB-84049-2.jpg

Immunofluorescence analysis of HeLa
cells using AMPK alpha 1 antibody. Blue: DAPI for nuclear
staining.

AB-84049-3.jpg

Review for AMPK alpha 1 Polyclonal Antibody
Application:Immunofluorescence (IF)
Sample: Human lung cancer cells
Loading Amount 15 ug
Dilution 1:50
Incubation time over night
Temperature: 4 ℃
Exposure: 30s
Remarks: Immunofluorescence analysis of Human lung cancer cells using antibody at 1:50 dilution. The positive signal is Golgi staining.

AB-84049-4.jpg

Review for AMPK alpha 1 Polyclonal Antibody
Application: Western blot (WB)
Sample: Human lung cancer cells
Loading Amount:15 ug
Dilution:1:1000
Incubation time: over night
Temperature: 4 ℃
Exposure:10s
Remarks: Western blot analysis of extracts of Human lung cancer cells, using antibody at 1:1000 dilution.

AB-84049-5.jpg

Review for AMPK alpha 1 Polyclonal Antibody
Application: Western blot (WB)
Sample: HeLa
Loading Amount:20 ug
Dilution: 1:1000
Incubation time: over night
Temperature: 4 ℃
Exposure: 15s
Observed Band: 62 kDa
Remarks: Western blot analysis of extracts from Hela cell using p-AMPK(S172) and AMPK Antibody (1:1000)
shATG5+:KD

References

References for AMPK alpha 1 Polyclonal Antibody
Product: AMPK alpha 1 Polyclonal Antibody
Journal: Biochemical Pharmacology
Application:WB IF: 4.580
Species: Human
PMID: 27816546
Title: Anhydroicaritin improves diet-induced obesity and hyperlipidemia and alleviates insulin resistance by suppressing SREBPs activation

References for AMPK alpha 1 Polyclonal Antibody
Product: AMPK alpha 1 Polyclonal Antibody
Journal: Journal of ethnopharmacology
Application:WB IF:2.980
Species:Rat
PMID:28993280
Title: Antidiabetic activities of entagenic acid in type 2 diabetic db/db mice and L6 myotubes via AMPK/GLUT4 pathway

References for AMPK alpha 1 Polyclonal Antibody
Product:AMPK alpha 1 Polyclonal Antibody
Journal:Oncology
Application:WB, IF: 2.262
Species:human
PMID:29566977
Title:CO suppresses prostate cancer cell growth by directly targeting LKB1/AMPK/mTOR pathway in vitro and in vivo

References for AMPK alpha 1 Polyclonal Antibody
Product: AMPK alpha 1 Polyclonal Antibody
Journal: Biomedicine & pharmacotherapy = Biomédecine & pharmacothérapie
Application:WB IF:3.45
Species:Mouse
PMID:30537674
Title: Novel hepatoprotective role of Leonurine hydrochloride against experimental non-alcoholic steatohepatitis mediated via AMPK/SREBP1 signaling pathway.