Anti-beta Actin Rabbit Polyclonal Antibody
| Cat Number: | ABS11001 |
|---|---|
| Conjugate: | unconjugated |
| Size: | 100 μL |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse beta-Actin |
| Reactivity: | Species, Human, Mouse, Rat |
| Applications: | WB 1: 1000-1: 2000 IHC/IF 1: 500-1: 1000 ICC/IF 1: 300-1: 1000 |
| Molecular: | 42 kDa |
| Purification: | Affinity purification |
| Synonyms: | ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin, β-Actin |
| Background: | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. |
| Form: | Liquid |
| Buffer: | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
| Storage: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
WB analysis of beta Actin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.
IF analysis ofbeta Actinred.Sample: Human colon cancer, 4% PFA 12-24h.Antigen retrieval: Tris-EDTA buffer , 98℃, 20min.Blocking buffer: 3% BSA in PBS, RT, 30min.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG , 1: 300, RT, 1h.
IF analysis ofbeta Actinred.Sample: Rat liver, 4% PFA 12-24h.Antigen retrieval: Tris-EDTA buffer , 98℃, 20min.Blocking buffer: 3% BSA in PBS, RT, 30min.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG , 1: 300, RT, 1h.