Anti-LC3A/B Rabbit pAb
| Cat Number: | ABS11124 |
|---|---|
| Conjugate: | unconjugated |
| Size: | 100 μL |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse LC3B |
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB 1: 1000-1: 2000 ICC/IF 1: 300-1: 600 |
| Molecular: | 14/16 kDa / 14/16 kDa |
| Purification: | Affinity purification |
| Synonyms: | LC3, LC3A, LC3A/B, LC3B, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, MAP1ALC3, MAP1BLC3, MAP1LC3A, MAP1LC3A/B, MAP1LC3B |
| Background: | LC3, also known as microtubule-associated protein 1 light chain 3 (MAP1LC3), is a mammalian homolog of the yeast Atg8p (autophagy-related 8 protein) that is involved in autophagy process. Three isoforms of LC3 exist as LC3A,LC3B, and LC3C. LC3 is first synthesized as pro-LC3, and undergoes C-terminal cleavage to yield a cytosolic precursor known as LC3-I. During autophagy LC3-I is subsequently conjugated to PE lipid as lipidated form: LC3-II. The lipidated form migrates faster on SDS-PAGE gel. The increased the level of LC3-II has been widely used as autophagy marker. This antibody recognizes both LC3-I and LC3-II. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
| Storage: | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
WB analysis of LC3A/B. Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 200, RT, 1h.
ICC analysis of LC3A/Bred. Sample: C6, methanol 10min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG , 1: 300, RT, 1h.
ICC analysis of LC3A/Bred. Sample: HeLa, methanol 10min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG , 1: 300, RT, 1h.