Anti-Myelin Basic Protein Rabbit Polyclonal Antibody
| Cat Number: | ABS11226 |
|---|---|
| Conjugate: | unconjugated |
| Size: | 100 μL |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Myelin Basic Protein |
| Reactivity: | Mouse, Rat |
| Applications: | Western Blot: 1: 500-1: 1000 Immunohistochemistry: 1: 300-1: 600 Immunofluorescence: 1: 300-1: 600 |
| Molecular: | 17/20 kDa |
| Purification: | Affinity purification |
| Synonyms: | MBP, entrez:4155, myelin basic protein |
| Background: | The classic group of MBP isoforms (isoform 4-isoform 13) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
| Storage: | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
WB analysis of Myelin Basic Protein . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.
IHC analysis of MBP . Sample: Rat striatum , 4% PFA 12-24h. Antigen retrieval: Citrate buffer ,98℃,20 min. Blocking buffer: 3% BSA in PBS , RT, 30min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 200 RT 1h.
IF analysis of MBP . Sample: Rat brain , 4% PFA 12-24h. Antigen retrieval: Citrate buffer ,98℃,20 min. Blocking buffer: 3% BSA in PBS , RT, 30min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG , 1: 300 RT, 1h.