Anti-TOMM20 Rabbit pAb
| Cat Number: | ABS111481 |
|---|---|
| Conjugate: | unconjugated |
| Size: | 100 μL |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant protein corresponding to Mouse TOMM20 |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1: 1000-1: 2000 Immunocitochemistry: 1:500-1:1000 Immunofluorescence: 1:500-1:1000 |
| Molecular: | 16 kDa |
| Purification: | Affinity purification |
| Synonyms: | None |
| Background: | TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms. |
| Form: | Liquid |
| Buffer: | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
| Storage: | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
WB analysis of .Tom20 . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.
ICC analysis of.Tom20 red. Sample: HeLa, 4% PFA 20min. Blocking buffer: Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG , 1: 300, RT, 1h..
ICC analysis of.Tom20 red. Sample: HepG2, 4% PFA 20min. Blocking buffer: Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG , 1: 300, RT, 1h..