APOA1 Rabbit Polyclonal Antibody
| Cat Number: | AB-84735 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 25-267 of human APOA1. |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot 1:100 – 1:500 |
| Molecular Weight: | 26kDa |
| Purification: | Affinity purification |
| Synonyms: | APOA1;apo(a) |
| Background: | This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This |
| Form: | Liquid |
| Buffer: | PBS with 0.05% proclin300,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using APOA1 antibody at 1:500dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 30s.
Immunohistochemistry of paraffin-embeddedmouse liver using APOA1 Rabbit pAb at dilution of 1:20 (40x lens). Perform highpressure antigen retrieval with 10 mM citratebuffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of HepG2 cellsusing APOA1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclearstaining.
Immunohistochemistry of paraffinembedded mouse brain using APOA1 antibody at dilution of 1:100 (40x lens).