Aquaporin 5 (AQP5) Rabbit Polyclonal Antibody
| Cat Number: | AB-90206 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 188-203 of human AQP5. |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1:1000 – 1:5000 |
| Molecular Weight: | 24 kDa |
| Purification: | Affinity purification |
| Synonyms: | PPKB; AQP-5 |
| Background: | Aquaporin 5 (AQP5) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions. AQP0, AQP2, AQP5, and AQP6 are closely related and all map to 12q13. |
| Form: | Liquid |
| Buffer: | PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of various lysates, using AQP5 Rabbit pAb at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 60s.
Immunofluorescence analysis of NIH/3T3 cells using AQP5 antibody at dilution of 1:100. Blue: DAPI for nuclear
staining.
Immunofluorescence analysis of U2OS cells using AQP5 antibody at dilution of 1:100.
Blue: DAPI for nuclear staining.