ASM
| Cat Number: | AB-J199 |
|---|---|
| Size: | 100ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant protein of human SMPD1 / ASM |
| Reactivity: | Hu, Ms |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 60kDa |
| Purification: | Aff. Pur. |
| Synonyms: | SMPD1;ASM;ASMASE;NPD;SMPD1/ASM |
| Background: | The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann- Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of THP-1 cells, using ASM antibody at 1:1000 dilution. THP-1 cells were treated by PMA
(80 nM) for overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus
Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using ASM antibody at
1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 30s.
Immunofluorescence analysis of NIH-3T3
cells using ASM Polyclonal
Antibody at dilution of 1:100
(40x lens).
Blue: DAPI for nuclear staining.