Cat Number: AB-82599
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant protein of human BECN1
Reactivity: Hu,Ms,Rt

WB: 1: 500-1:1000 Immunohistochemistry araffin Embedded tissues 1: 100
Immunofluorescence: 1:100

Molecular Weight: 60 kDa
Purification: Aff. Pur.
Synonyms: ATG6;VPS30;beclin1

This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.

Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at +4°C for short term, at -20° C for 12 months. Avoid Freeze and Thaw Cycles.

Western blot analysis of extracts from normal (control) and BECN1 knockout (KO) 293T cells, using BECN1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.


Immunohistochemistry of paraffin-embedded human breast cancer using BECN1 antibody at dilution of 1:100 (40x lens).


Immunofluorescence analysis of HeLa cells using BECN1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.