BECLIN 1
| Cat Number: | AB-82599 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant protein of human BECN1 |
| Reactivity: | Hu,Ms,Rt |
| Applications: | WB: 1: 500-1:1000 Immunohistochemistry araffin Embedded tissues 1: 100 |
| Molecular Weight: | 60 kDa |
| Purification: | Aff. Pur. |
| Synonyms: | ATG6;VPS30;beclin1 |
| Background: | This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at +4°C for short term, at -20° C for 12 months. Avoid Freeze and Thaw Cycles. |
Western blot analysis of extracts from normal (control) and BECN1 knockout (KO) 293T cells, using BECN1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human breast cancer using BECN1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using BECN1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.