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beta2- Microglobulin

Cat Number: AB-84271
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human beta 2 Microglobulin
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 – 1:2000
Immufluorescence: 1:50 – 1:200

Molecular: 12kDa
Purification: Aff. Pur.
Synonyms: B2M;IMD43
Background:

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-84271-1.jpg

Western blot analysis of extracts of
various cell lines, using beta 2
Microglobulin antibody at
1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.

AB-84271-2.jpg

Immunofluorescence analysis of
MCF-7 cells using beta 2
Microglobulin antibody
Blue: DAPI for nuclear staining.