C-Myc
| Cat Number: | MAB-94579 |
|---|---|
| Size: | 100μg |
| Clone: | MYC |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant protein of human c-Myc |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 49kDa |
| Purification: | Aff. Pur. |
| Synonyms: | MRTL;MYCC;bHLHe39;c-Myc;MYC |
| Background: | The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt’s lymphomas, suggesting its importance in the normal function of this gene. |
| Form: | Liquid |
| Buffer: | Store at -20℃. Avoid freeze / thaw cycles |
| Storage: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
Western blot analysis of MYC using anti-MYC antibody in 1) Jurkat cell lysate;(2) K562 cell lysate..
The membrane was incubated with rabbit anti-MYC antigen affinity purified polyclonal antibody at 1ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an ECL West Pico Plus. A specific band was detected for MYC.
IHC analysis of MYC using anti-MYC antibody on paraffin-embedded human lung cancer.
Immunofluorescent analysis of HeLa cells,
using c-Myc Antibody.