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c-Src Rabbit Polyclonal Antibody

Cat Number: AB-84358
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: The antiserum was produced against synthesized peptide derived from human Src. AA range:487-536
Reactivity: Human;Mouse;Rat
Applications:

Western Blot: 1/500 – 1/2000.
Immunohistochemistry: 1/100 – 1/300

Immunoprecipitation  1:50-200

Molecular Weight: 59kD
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms: SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src
Background:

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase.Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
AB-84358-1

Western Blot analysis of various cells using c-Src Polyclonal Antibody diluted at 1:2000

AB-84358-2

Western Blot analysis of HeLa cells using c-Src Polyclonal Antibody diluted at 1:2000

AB-84358-3

Western Blot analysis of hela using c-Src Polyclonal Antibody. Antibody was diluted at 1:2000

AB-84358-4

Western Blot analysis of HELA HEPG2 using c-Src Polyclonal Antibody diluted at 1:2000