CADHERIN-17/ LI Cadherin Rabbit Monoclonal Antibody
| Cat Number: | MAB-94763 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | ARC1989 |
| Concentration: | 1 mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LI Cadherin/Cadherin-17 |
| Reactivity: | Human; Mouse; Rat |
| Applications: | Western Blot: 1:500 – 1:2000 Immunocytochemistry: 1:50 – 1:200 |
| Molecular Weight: | 120 kDa |
| Purification: | Affinity purification |
| Synonyms: | CDH16; HPT-1; HPT1 |
| Background: | This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using LI Cadherin/Cadherin-17 Rabbit mAb
at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 90s.
Immunohistochemistry of paraffinembedded human colon carcinoma using LI Cadherin/Cadherin-17 Rabbit mAb at dilution of 1:100 (40x
lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffinembedded human appendix using LI Cadherin/Cadherin-17 Rabbit mAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
mmunofluorescence analysis of rat rectum using LI Cadherin/Cadherin-17 Rabbit mAb at dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse large intestine using LI Cadherin/Cadherin-17 Rabbit mAb
at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.