Cadherin-N
| Cat Number: | AB-82904 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 750-850 of human N-Cadherin |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot, WB (1: 500- 1:1,000) |
| Purification: | Purified |
| Synonyms: | CDH2; CD325; CDHN; CDw325; NCAD; cadherin-2 |
| Background: | This gene encodes a classical cadherin and member of the cadherin superfamily.Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. |
| Form: | Liquid |
| Storage: | 2-8°C for short term for longer term at -20°C for one year.Avoid repeated freeze and thaw cycles. |
Western blot analysis of extracts of various cell lines, using N-Cadherin antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse liver using N-Cadherin Antibody at dilution of 1:100