Caspase 3 Rabbit Polyclonal antibody
| Cat Number: | AB-83835 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant protein of human CASP3 |
| Reactivity: | Human , Mouse , Rat |
| Applications: | Western Blot: 1:1000 Immunohistochemistry:1:100 Immunofluorescence: 1:50 - 1:100 Immunoprecipitation: 1:50 - 1:100 |
| Molecular Weight: | Casp3: 35kDa - cleaved Casp3: 17kDa |
| Purification: | Affinity purification |
| Background: | Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2). |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Storage: | Store at -20°C (regular) Avoid freeze / thaw cycles. |