Caspase 3 Rabbit Polyclonal antibody
| Cat Number: | AB-83835 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant protein of human CASP3 |
| Reactivity: | Human , Mouse , Rat |
| Applications: | Western Blot: 1:1000 Immunohistochemistry:1:100 Immunofluorescence: 1:50 – 1:100 |
| Molecular Weight: | Casp3: 35kDa - cleaved Casp3: 17kDa |
| Purification: | Affinity purification |
| Background: | Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2). |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Storage: | Store at -20°C (regular) Avoid freeze / thaw cycles. |
Western blot analysis of extracts of Jurkat cells at 1:1000 dilution. Jurkat cells treated by Etoposide 25uM etoposide for 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Pico Exposure time: 3s. AB83240 can detect both the full length and the cleaved form.
Immunohistochemistry of paraffin-embedded human tonsil using CASP3 antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded rat brain using CASP3 antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human placenta using CASP3 antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human gastric cancer using CASP3 antibody at dilution of 1:100
Immunofluorescence analysis of C6 cells using CASP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.