Cathepsin L Rabbit Monoclonal Antibody
| Cat Number: | MAB-95047 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | ARC59023 |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human Cathepsin L |
| Reactivity: | Human |
| Applications: | Western Blot: 1:500 – 1:1000 |
| Molecular Weight: | 42kDa |
| Purification: | Affinity purification |
| Synonyms: | MEP; CATL; CTSL1; Cathepsin L |
| Background: | The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Additionally, this protein cleaves the S1 subunit of the SARS-CoV-2 spike protein, which is necessary for entry of the virus into the cell. |
| Form: | Liquid |
| Buffer: | PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of various lysates, using Cathepsin L/V/K/H Rabbit mAbat 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Cathepsin L/V/K/H Rabbit mAb at dilution of 1:100 (40x lens). High pressure
antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.