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CCR2

Cat Number: AB-84344
Conjugate: Unconjugated
Size: 100ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthetic peptide of human CCR2.
Reactivity: Hu, Ms
Applications:

Western Blot: 1:500 – 1:2000
Immunofluorescence: 1:50 – 1:200

Molecular: 41kDa
Purification: Aff. Pur.
Synonyms: CCR2;CC-CKR-2;CCR-;CCR2A;CCR2B;CD192;CKR2;CKR2A;CKR2B;CMKBR2;MCP-1-R;FLJ78302
Background:

This gene encodes two isoforms of a receptor for monocyte chemoattractant protein-1, a chemokine which specifically mediates monocyte chemotaxis. Monocyte chemoattractant protein-1 is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the inflammatory response against tumors. The receptors encoded by this gene mediate agonist-dependent calcium mobilization and inhibition of adenylyl cyclase. This gene is located in the chemokine receptor gene cluster region. Two alternatively spliced transcript variants are expressed by the gene.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-84344-1.jpg

Western blot analysis of extracts of A-431 cells, using CCR2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL West Pico Plus.
Exposure time: 90s.

AB-84344-2.jpg

Immunofluorescence analysis of THP-1cells using CCR2 Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for
nuclear staining.