CD100/SEMA42
| Cat Number: | AB-84214 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 753-862 of human SEMA4D. |
| Reactivity: | Hu |
| Applications: | Western Blot: 1:200 – 1:1000 |
| Molecular Weight: | 150kDa |
| Purification: | Aff. Pur. |
| Synonyms: | SEMA4D;C9orf164;CD100;M-sema-G;SEMAJ;coll- |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of
293T cells, using SEMA4D antibody
at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.