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CD19 Rabbit Polyclonal Antibody

Cat Number: AB-84754
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: Synthesized peptide derived from B-lymphocyte antigen CD19 at AA range: 191-240
Reactivity: Human, Mouse, Rat
Applications:

Western Blot: 1:500-2000
Flow Cytometry: 1:50-200
Immunohistochemistry(paraffin-embedded tissues): 1:100-500
ELISA: 1:5000-20000

Molecular Weight: 61 kDa
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms: CD19; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD19
Background:

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the
threshold for antigen receptor-dependent stimulation.

Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
AB-84754-1

Western Blot analysis of mouse brain cells using CD19 Polyclonal Antibody. Antibody was diluted at 1:2000.
Secondary antibody was diluted at 1:20000

AB-84754-2

Western Blot analysis of MOUSE-BRAIN cells using CD19 Polyclonal Antibody diluted at 1:2000. Secondary
antibody was diluted at 1:20000

AB-84754-3

Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:100