CD2 Rabbit Monoclonal Antibody
| Cat Number: | MAB-94776 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | ARC59359 |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant protein of human CD2. |
| Reactivity: | Human |
| Applications: | Western Blot: 1:500 – 1:1000 |
| Molecular Weight: | 40-65 kDa |
| Purification: | Affinity purification |
| Synonyms: | T11; SRBC; LFA-2 |
| Background: | The protein encoded by this gene is a surface antigen found on all peripheral blood Tcells. The encoded protein interacts with LFA3 (CD58) on antigen presenting cells to optimize immune recognition. A locus control region (LCR) has been found in the 3′ flanking sequence of this gene. |
| Form: | Liquid |
| Buffer: | PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of various lysates, using CD2 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 180s.
Immunohistochemistry of paraffinembedded human tonsil using CD2 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.