CD2 Rabbit Monoclonal Antibody

Cat Number: MAB-94776
Conjugate: Unconjugated
Size: 100 ug
Clone: ARC59359
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: Recombinant protein of human CD2.
Reactivity: Human

Western Blot: 1:500 – 1:1000
Immunohistochemistry (paraffin-embedded tissues) 1:50 – 1:200

Molecular Weight: 40-65 kDa
Purification: Affinity purification
Synonyms: T11; SRBC; LFA-2

The protein encoded by this gene is a surface antigen found on all peripheral blood Tcells. The encoded protein interacts with LFA3 (CD58) on antigen presenting cells to optimize immune recognition. A locus control region (LCR) has been found in the 3′ flanking sequence of this gene.

Form: Liquid
Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Western blot analysis of various lysates, using CD2 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 180s.


Immunohistochemistry of paraffinembedded human tonsil using CD2 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.