CD3 epsilon Rabbit Polyclonal Antibody
| Cat Number: | AB-81831 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | RThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Reactivity: | Hu,Ms,Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 21kDa |
| Purification: | Aff. Pur. |
| Synonyms: | CD3E;IMD18;T3E;TCRE;CD3e molecule |
| Background: | The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11.The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of
various cell lines, using CD3E
Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus
Exposure time: 5s
Immunohistochemistry of paraffin embedded
human tonsil using CD3E
Antigen antibody at dilution
of 1:200 (40x lens).