CD3 epsilon (activation epitope) Mouse Monoclonal Antibody
| Cat Number: | MAB-94775 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | APA1/1 |
| Concentration: | 1mg/ml |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Purified human CD3 proteins isolated from thymus |
| Reactivity: | Mouse, Human |
| Applications: | Flow cytometry: 1-4 μg/ml; positive control: human T cells stimulated with anti-CD3 (MEM-57) antibody (1 μg/ml). Immunocytochemistry: Fixed and permeabilised cells. The antibody can distinguish TCR-stimulated from non-stimulated cells. Immunoprecipitation Western blot Immunohistochemistry (frozen tissues) |
| Purification: | Purified by protein-A affinity chromatography. |
| Synonyms: | CD3 epsilon chain, T3E, IMD18 |
| Background: | Sample preparation: At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1% BSA for 20 min. on ice. Wash with PBS and fix with 2% formaldehyde, 30 min on ice. Wash with PBS and incubate in PBS + 0,1% saponine, 5 min. RT. Incubate the cells in PBS + 1% BSA + 0.03% saponine, 15 min. on ice. Incubate with fluorescence-labeled APA1/1 antibody (1-2 μg/ml) in PBS + 1% BSA + 0.03% saponine in dark, 20 min. RT. Wash with PBS + 1% BSA + 0.03% saponine, resuspend in PBS. |
| Form: | Liquid |
| Buffer: | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Storage: | Store at 2-8°C. Do not freeze. |