Top

CD31 / PECAM1

Cat Number: AB-83615
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of mouse CD31
Reactivity: Hu,Ms,Rt
Applications:

Western Blot: 1:500 – 1:2000
Immunohistochemistry: 1:50 – 1:200
Immunoprecipitation: 1:50 – 1:200
Immunofluorescence: 1:50-1:200

Molecular: 130kDa
Purification: Aff. Pur.
Synonyms: PECAM1;CD31;CD31/EndoCAM;GPIIA;PECA1;PECAM-1;e ndoCAM
Background:

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-83615-1.jpg

Western blot analysis of extracts of various cell lines, using CD31
antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 3s.

AB-83615-2.jpg

Immunohistochemistry of paraffin embedded
human esophagus using
CD31 antibody at dilution of
1:100 (40x lens).

AB-83615-3.jpg

Immunofluorescence analysis of
HUVEC cells using CD31 antibody
at dilution of 1:100.
Blue: DAPI for nuclear staining.

AB-83615-4.jpg

Immunofluorescence analysis of
U2OS cells using CD31 antibody
at dilution of 1:100.
Blue: DAPI for nuclear staining.

AB-83615-5.jpg

Review for CD31 Rabbit Polyclonal Antibody

Experiment Type: Immunofluorescence (IF)
Sample: Rat lung

AB-83615-6.jpg

Review for CD31 Rabbit Polyclonal Antibody

Experiment Type:
Immunohistochemistry(IHC)

Sample: Human prostate
Description: Immunohistochemistry analysis of Human prostate, using PECAM1 Polyclonal Antibody.