CD31 / PECAM1 Rabbit Polyclonal Antibody
| Cat Number: | AB-83615 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of mouse CD31 |
| Reactivity: | Hu,Ms,Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 130kDa |
| Purification: | Aff. Pur. |
| Synonyms: | PECAM1;CD31;CD31/EndoCAM;GPIIA;PECA1;PECAM-1;e ndoCAM |
| Background: | The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using CD31
antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 3s.
Immunohistochemistry of paraffin embedded
human esophagus using
CD31 antibody at dilution of
1:100 (40x lens).
Immunofluorescence analysis of
HUVEC cells using CD31 antibody
at dilution of 1:100.
Blue: DAPI for nuclear staining.
Immunofluorescence analysis of
U2OS cells using CD31 antibody
at dilution of 1:100.
Blue: DAPI for nuclear staining.
Review for CD31 Rabbit Polyclonal Antibody
Experiment Type: Immunofluorescence (IF)
Sample: Rat lung
Review for CD31 Rabbit Polyclonal Antibody
Experiment Type:
Immunohistochemistry(IHC)
Sample: Human prostate
Description: Immunohistochemistry analysis of Human prostate, using PECAM1 Polyclonal Antibody.