CD324 / E-cadherin Mouse Monoclonal Antibody
| Cat Number: | MAB-93240 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100ug |
| Clone: | 67A4 |
| Concentration: | 1mg/ml |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | T-47D cells |
| Reactivity: | Human |
| Applications: | Western Blot, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry(paraffin-embedded tissues), Flow Cytometry Working dilutions: Immunohistochemistry (frozen sections):4-8 μg/ml |
| Purification: | Purified by protein-A affinity chromatography. |
| Synonyms: | Cadherin-1, E-cadherin, Uvomorulin, CAM 120/80, UVO, CDHE, ECAD, LCAM, BCDS1, Arc-1 |
| Background: | The mouse monoclonal antibody 67A4 recognizes an extracellular epitope of CD324 / E-cadherin, an approximately 100 kDa epithelial cell adhesion molecule, whose detection is important for determination of invasive potential of epithelial neoplasms. |
| Form: | Liquid |
| Buffer: | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide. |
| Storage: | Store at 2-8°C. Do not freeze. |
Western blot analysis of extracts of HCT116 cells, using CDH1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 180s.