CDK4 Rabbit Polyclonal Antibody
| Cat Number: | AB-80582 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100μg |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the N-terminal region of human CDK4. AA range:1-50 |
| Reactivity: | Human;Rat;Mouse |
| Applications: | Western Blot: 1/500 – 1/2000 Immunfluorescence: 1:50-200 Immunohistochemistry (paraffin embedded tissues): 1:100-300 |
| Molecular Weight: | 35kDa |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Synonyms: | CDK4; Cyclin-dependent kinase 4; Cell division protein kinase 4; PSK-J3 |
| Background: | cyclin dependent kinase 4(CDK4) Homo sapiens The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western Blot analysis of MCF7, K562 cells using Cdk4 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
Immunofluorescence analysis of Hela cell. 1,Cdk4 Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature,50min). 3 DAPI(blue) 10min.
Immunofluorescence analysis of human-stomach tissue.1,Cdk4 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody
was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-lung tissue. 1,Cdk4 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody
was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B
Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100
Immunohistochemical analysis of paraffin-embedded rat-muscle, antibody was diluted at 1:100