CDKN1B
| Cat Number: | AB-82085 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-198of human CDKN1B/p27KIP1 (NP_004055.1). |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 27kDa |
| Purification: | Aff. Pur. |
| Synonyms: | CDKN1B;CDKN4;KIP1;MEN1B;MEN4;P27KIP1;p27 KIP 1 |
| Background: | This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts from normal (control) and CDKN1B/p27KIP1 knockout (KO) HeLa cells, using CDKN1B/p27KIP1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 3min.
Western blot analysis of extracts of various cell lines, using CDKN1B/p27KIP1 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 3min.
Immunohistochemistry of paraffin embedded rat brain using CDKN1B/p27KIP1 Antibody at
dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin embedded
mouse kidney using
CDKN1B/p27KIP1 Antibody at
dilution of 1:200 (40x lens).
Immunofluorescence analysis of NIH/3T3 cells using p27 KIP 1 antibody at dilution of 1:100.
Blue: DAPI for nuclear
staining.
Review for CDKN1B/p27KIP1 Rabbit Polyclonal Antibody
Experiment Type: Western blot(WB)
References
References for CDKN1B/p27KIP1 Rabbit pAb
Product: CDKN1B/p27KIP1 Rabbit pAb
Journal: Journal of agricultural and food chemistry
Application: WB
IF: 3.41
Species: Homo sapiens
PMID: 30507186
Title: Nobiletin down-regulates c-KIT gene expression and exerts anti-leukemic effects on human acute myeloid leukemia cells.
References for CDKN1B/p27KIP1 Rabbit pAb
Product:CDKN1B/p27KIP1 Rabbit pAb
Journal:Experimental and Molecular Medicine
Application:WB, IHC
IF:5.061
Species:Mus musculus
PMID:28642576
Title:Upregulation of miR-223 in the rat liver inhibits proliferation of hepatocytes under simulated microgravity
References for CDKN1B/p27KIP1 Rabbit pAb
Product:CDKN1B/p27KIP1 Rabbit pAb
Journal:Cells
Application:WB
IF:5.65
Species:Homo sapiens
PMID:32260160
Title:Nobiletin Promotes Megakaryocytic Differentiation through the MAPK/ERK-Dependent EGR1 Expression and Exerts Anti-Leukemic Effects in Human Chronic Myeloid Leukemia (CML) K562 Cells
References for CDKN1B/p27KIP1 Rabbit pAb
Product:CDKN1B/p27KIP1 Rabbit pAb
Journal:Journal of Virology
Application:WB
IF:4.324
Species:Homo sapiens
PMID:27334596
Title:BZLF1 Attenuates Transmission of Inflammatory Paracrine Senescence in Epstein-Barr Virus-Infected Cells by Downregulating Tumor Necrosis Factor Alpha.