cGAS Rabbit Monoclonal Antibody
Cat Number: | MAB-94713 |
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Conjugate: | Unconjugated |
Size: | 100 ug |
Clone: | D1D3G |
Concentration: | 1mg/ml |
Host: | Rb |
Isotype: | IgG |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-522 of human cGAS |
Reactivity: | Hu, Ms, Rt |
Applications: | WB 1:500 – 1:2000 |
Molecular Weight: | 62KDa |
Purification: | Aff. Pur. |
Synonyms: | MB21D1;C6orf150;cGAS;h-cGAS;CGAS |
Background: | Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP |
Form: | Liquid |
Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using cGAS antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Immunohistochemistry of paraffinembedded human colon using Cgas Rabbit MAb at dilution of 1:50 (40x lens)
Immunofluorescence analysis of PC-12 cells using cGAS Rabbit MAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.