cGAS Rabbit Monoclonal Antibody

Cat Number: MAB-94713
Conjugate: Unconjugated
Size: 100 ug
Clone: D1D3G
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-522 of human cGAS
Reactivity: Hu, Ms, Rt

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50-1:100 ICC 1:50 – 1:100

Molecular Weight: 62KDa
Purification: Aff. Pur.
Synonyms: MB21D1;C6orf150;cGAS;h-cGAS;CGAS

Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP
and GTP and plays a key role in innate immunity. Catalysis involves both the formation
of a 2′,5′ phosphodiester linkage at the GpA step and the formation of a 3′,5′
phosphodiester linkage at the ApG step, producing c[G(2′,5′)pA(3′,5′)p] . Acts as a key
cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm
being a danger signal that triggers the immune responses . Binds cytosolic DNA directly,
leading to activation and synthesis of cGAMP, a second messenger that binds to and
activates TMEM173/STING, thereby triggering type-I interferon production.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Western blot analysis of extracts of various cell lines, using cGAS antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.


Immunohistochemistry of paraffinembedded human colon using Cgas Rabbit MAb at dilution of 1:50 (40x lens)


Immunofluorescence analysis of PC-12 cells using cGAS Rabbit MAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.