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Cleaved-Caspase-7 p20 (Asp-198)

Cat Number: AB-J532
Size: 100ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Synthesized peptide derived from human Caspase-7 p20
Reactivity: Hu, Ms
Applications:

Western Blot: 1:500-1:2000
Immunohistochemistry: 1:100-1:300
ELISA: 1:5000

Molecular Weight: 20kDa
Purification: The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
Synonyms: Caspase-7 CASP-7 Apoptotic protease Mch-3 CMH-1 ICE-like apoptotic protease 3 ICE-LAP3 Caspase-7 subunit p20 Caspase-7 subunit p11
Background:

Specificity: Cleaved-Caspase-7 p20 (Asp198) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-7 p20 protein resulting from cleavage adjacent to Asp198. Tissue Specificity: Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.

Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20°C, and avoid repeat freeze-thaw cycles.
AB-J532-1.jpg

Western Blot (WB) analysis
of specific cells using
Cleaved-Caspase-7 p20 (Asp198) polyclonal antibody.

AB-J532-2.jpg

Western blot analysis of lysates from Jurkat cells, treated with Etoposide 25uM 24h, using Cleaved -Caspase 7 p20 (Asp198) Antibody. The lane is blocked with the synthesized peptide.

AB-J532-3.jpg

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Cleaved-Caspase 7 p20(Asp198) Antibody. The picture is blocked with the synthesized peptide.