Cleaved PARP-1 (D214) Rabbit Polyclonal Antibody
| Cat Number: | AB-84744 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PARP. AA range:165-214 |
| Reactivity: | Human |
| Applications: | Western Blot: 1:500-2000 Immunofluorescence: 1:50-300 Immunohistochemistry: 1:50-300 |
| Molecular Weight: | 24 kDa |
| Purification: | The antibody was affinity- purified from rabbit antiserum by affinity- chromatography using epitope- specific immunogen. |
| Synonyms: | PARP1; ADPRT; PPOL; Poly [ADP-ribose] polymerase 1; PARP- 1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly [ADP-ribose] synthase 1 |
| Background: | poly(ADP-ribose) polymerase 1(PARP1) Homo sapiens This gene encodes a chromatin-associated enzyme, poly( ADP- ribosyl) transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Western blot analysis of lysates from A549 cells, treated with etoposide 25uM 24h, using PARP (Cleaved-Asp214) Antibody.
The lane on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of Human-stomach-cancer tissue. 1,Cleaved-PARP-1 (D214) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target.
Picture B: DAPI.
Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-colon-cancer tissue. 1,Cleaved-PARP-1 (D214) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.