COLLAGEN II / COL2A1 Rabbit Polyclonal Antibody
| Cat Number: | AB-84753 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Collagen II. AA range:101-150 |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1/500 – 1/2000 |
| Molecular Weight: | 140 kDa |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Synonyms: | COL2A1; Collagen alpha-1(II) chain; Alpha-1 type II collagen |
| Background: | This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Western blot analysis of lysates from 1) k562 , 2) COLO205,3) MCF-7 cells,
Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody was diluted at 1:10000, 37° 1hour.
Red） Actin β Monoclonal Antibody(5B7)
Immunofluorescence analysis of rat-lung tissue.
1,COL2A1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight).
2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).
3, Picture B: DAPI(blue) 10min.
Picture A:Target.
Picture B: DAPI.
Picture C: merge of A+B
Immunofluorescence analysis of rat-lung tissue.
1,COL2A1 Polyclonal Antibody(red) was diluted at
1:200(4°C,overnight).
2, Cy3 labled Secondary antibody
was diluted at 1:300(room temperature, 50min).
3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture
Immunofluorescence analysis of rat-spleen tissue.
1,COL2A1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight).
2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).
3, Picture B: DAPI(blue) 10min.
Picture A:Target.
Picture B: DAPI.
Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,COL2A1 Polyclonal Antibody was
diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 ° C,20min).
3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by
secondary antibody only.