Complement C3 α-chain Mouse Monoclonal Antibody

Cat Number: MAB-94388
Conjugate: Unconjugated
Size: 100 ug
Clone: 6E8
Concentration: 1mg/ml
Host: Mouse
Isotype: IgG1
Immunogen: Recombinant netrin domain of human C3
Reactivity: Human

Western Blot: 1:1,000-1:5,000

Molecular Weight: 185kDa
Purification: Purified

Complement component 3, often simply called C3, is the third protein in the complement system. The complement system “complements” the ability of antibodies and phagocytic cells to clear pathogens such as bacteria and viruses from the organism and can trigger inflammation and remove debris from cells and tissues. C3 is involved in both the classical and alternative pathway. The
MCA-6E8 antibody binds to the netrin-like domain in the α subunit of human C3.

Form: Liquid
Buffer: Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Storage: Store at 4°C for short term, for longer term at -20°C

Western blot analysis of 6E8 on human serum samples. 0.1μg purified human C3 protein (lane 1) and 10 μg normal human serum protein (lane 2) were blotted with Complement C3 α-chain at a concentration 1:3,000. The Complement C3 α-chain monoclonal binds strongly and cleanly to a band at about 115kDa which represents the intact α subunit of C3 and a weaker proteolytic band at approximately 40kDa. Bands at 190kDa and above are likely the pro-C3 and its glycosylated form.