Connexin 43
| Cat Number: | AB-90007 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequencem corresponding to amino acids 233-382 of human Connexin43. |
| Reactivity: | Hu, Rt, Ms |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 38kDa |
| Purification: | Aff. Pur. |
| Synonyms: | GJA1;AVSD3;CMDR;CX43;EKVP;GJAL;HLHS1;HSS;ODDD; PPKCA;EKVP3 |
| Background: | This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol ,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of
various cell lines, using GJA1 antibody
at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.
Immunofluorescence analysis of
mouse brain using Connexin43 Polyclonal
Antibody at dilution of1:100 (40x lens).
Blue: DAPI for
nuclear staining.