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CSPG4

Cat Number: AB-84322
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1950-2210 of human CSPG4.
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 – 1:2000
Immunofluorescence: 1:50 – 1:200
Molecular Weight: 320kDa
Purification: Aff. Pur.
Synonyms: CSPG4;HMW-MAA;MCSP;MCSPG;MEL-CSPG;MSK16;NG2
Background:

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-84322-1.jpg

Western blot analysis of extracts of various cell lines, using CSPG4 antibody at 1:1000
dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 90s.

AB-84322-2.jpg

Western blot analysis of extracts from normal (control) and CSPG4 knockout (KO) HeLa cells, using CSPG4 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.

References

References for [KO Validated] CSPG4 Rabbit pAb
Product:[KO Validated] CSPG4 Rabbit pAb
Journal:Nature
Application:WB, IF
IF:43.07
Species:Mus musculus
PMID:32581380
Title:Reversing a Model of Parkinson’s Disease With in Situ Converted Nigral Neurons