CTGF
| Cat Number: | AB-84282 |
|---|---|
| Size: | 100ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western blotting: 1:1000 |
| Molecular Weight: | 35kDa |
| Purification: | Polyclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val174 of human CTGF protein. |
| Background: | Connective tissue growth factor (CTGF, CCN2) belongs to the CCN (CYR61, CTGF, NOV) family of secreted extracellular matrix (ECM) proteins (1). Members of this family contain four conserved cysteine-rich domains, and interact in the ECM with a diverse array of cell surface receptors, includ-ing integrins and heparin-sulfate proteoglycans (2). These interactions regulate a multitude of cellular and tissue functions, including adhesion, proliferation, migration, differentiation, senescence, angiogenesis, inflammation, and wound repair (1, 3-5). The CTGF gene is a transcriptional target of both YAP/TAZ and TGFβ-SMAD signaling pathways (6,7), and aberrant regula-tion of CTGF expression is strongly associated with pathological conditions, notably cancer and fibrosis (8, 9). CTGFRabbit pAb recognizes endogenous levels of total CTGF protein. By Western blot analysis, the antibody detects a 120 kDa protein of unknown identity in both CTGF-positive and CTGF-negative cell lines. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide and 50% glycerol pH 7.3 |
| Storage: | T -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
Western blot analysis of extracts from various cell
lines using CTGF Rabbit pAb (upper) and β-actin
(D6A8) Rabbit B Actin lower). Asterisk indicates a
120 kDa band of unknown identity detected by this
antibody in Western blot analysis.
Immunohistochemistry of
paraffin-embedded human
colon cancer tissue slide using
CTGF Antibody at dilution of
1:200 heat mediated antigen
retrieved with Tris-EDTA
buffer (pH9) .
Mouse heart tissue
were subjected to
SDS PAGE followed
by western blot with
CTGF Antibody)at
dilution of 1:600
References
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55.
(3) Hall-Glenn, F. and Lyons, K.M. (2011) Cell Mol Life Sci 68,
3209-17.
(4) Hashimoto, G. et al. (2002) J Biol Chem 277, 36288-95.
(5) Ivkovic, S. et al. (2003) Development 130, 2779-91.
(6) Di Benedetto, A. et al. (2016) Oncotarget 7, 43188-43198.
(7) Liu, H. et al. (2015) Cell Biochem Biophys 73, 775-81.
(8) Brigstock, D.R. (2010) J Cell Commun Signal 4, 1-4.
(9) Jun, J.I. and Lau, L.F. (2011) Nat Rev Drug Discov 10, 945-
63.