CXCR1
| Cat Number: | AB-82066 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 μg |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 250-350 of human CXCR1. |
| Reactivity: | Hu |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 70kDa |
| Purification: | Aff.Pur. |
| Synonyms: | CXCR1;C-C;C-C-CKR-1;CD128;CD181;CDw128a;CKR-1; CMKAR1;IL8R1;IL8RA;IL8RBA |
| Background: | The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. Knockout studies in mice suggested that this protein inhibits embryonic oligodendrocyte precursor migration in developing spinal cord. This gene, IL8RB, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36 |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using CXCR1
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L)at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus.
Exposure time: 15s.
Immunohistochemistry of paraffin embedded
rat lung using CXCR1
antibody at dilution of
1:100 (40x lens).
Immunohistochemistry of paraffin embedded mouse spleen using CXCR1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffinembedded
human colon using CXCR1
antibody at dilution of 1:100
(40x lens).