Cyclin B1 Rabbit Polyclonal Antibody
| Cat Number: | AB-83789 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Cyclin B1. AA range:91-140 |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1/500 – 1/2000 |
| Molecular Weight: | 60kD |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Background: | The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20℃. Avoid repeated freeze-thaw cycles. |
Western Blot analysis of PC-12 cells using Cyclin B1 Polyclonal Antibody diluted at 1:500
Western Blot analysis of various cells using Cyclin B1 Polyclonal Antibody diluted at 1:500
Western blot analysis of lysates from K562 cells, treated with serum 10% 15′, using Cyclin B1 Antibody.
The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue.
1,Cyclin B1 Polyclonal Antibody was diluted at 1:200(4°C,overnight).
2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min).
3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue.
1,Cyclin B1 Polyclonal Antibody was diluted at 1:200(4°C,overnight).
2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min).
3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue.
1,Cyclin B1 Polyclonal Antibody was diluted at 1:200(4°C,overnight).
2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min).
3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Negative control was used by secondary antibody only.
Immunofluorescence analysis of Mouse-kidney tissue.
1,Cyclin B1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight).
2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min)
3,Picture B: DAPI(blue) 10min.
Picture A:Target.
Picture B: DAPI.
Picture C: merge of A+B
Immunofluorescence analysis of Rat-liver tissue.
1,Cyclin B1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight).
2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).
3, Picture B: DAPI(blue) 10min.
Picture A:Target.
Picture B: DAPI.
Picture C: merge of A+B
Immunofluorescence analysis of HeLa cells, using Cyclin B1 Antibody. The picture on the right is blocked with the synthesized peptide.