DDDDK-Tag Mouse Monoclonal Antibody
| Cat Number: | MAB-94419 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | FR10 |
| Concentration: | 1mg/mL |
| Host: | Mouse |
| Isotype: | IgG1,Kappa |
| Immunogen: | A synthetic peptide corresponding to DDDDK tag. |
| Reactivity: | All Species |
| Applications: | Western Blot: 1:2000 – 1:5000 |
| Molecular Weight: | 55kDa/48kDa/40kDa |
| Purification: | Purified |
| Background: | FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting. |
| Form: | Liquid |
| Buffer: | PBS with 0.05% proclin300,50% glycerol,pH7.3. |
| Storage: | At +4°C for short term. For longer term store at -20°C, avoid repeat freeze-thaw cycles. |
Immunoprecipitation analysis of 200ug extract cell lysate from 293T cells transfected with GSK3B expression vector containing a DDDDK-Tag with 3 μg Mouse anti DDDDK-Tag mAb antibody.Western blot was performed from the immunoprecipitate using Mouse anti DDDDK-Tag mAb antibody.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with Renin
using Mouse anti DDDDK-Tag mAb at 1:5000 dilution.
Secondary antibody:HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 60s.
Immunofluorescence analysis of 293T cells using Mouse anti DDDDK-Tag mAb at dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.
Immunofluorescence analysis of 293TBCAT2- Flag-GFP-C cells using Mouse anti DDDDK-Tag mAb at dilution of
1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of 293TBCAT2- Flag-GFP-N cells using Mouse anti DDDDK-Tag mAb at dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.