DNA-PKCS
| Cat Number: | AB-83872 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide of human DNA-PKCS |
| Reactivity: | Hu, Ms |
| Applications: | Western Blot: 1:500 -1:2000 |
| Molecular Weight: | 469kDa |
| Purification: | Aff. Pur. |
| Synonyms: | DNA-PKCS; DNA-PKcs; DNAPK; DNPK1; HYRC; HYRC1; IM D26; XRCC7; p350; DNA-dependent protein kinase catalytic subunit |
| Background: | This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNAPK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using DNA-PKCS antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using DNA-PKCS antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using DNA-PKCS antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2OS cells using DNA-PKCS antibody at dilution of 1:100. Blue: DAPI for nuclear staining.