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DNA-PKCS

Cat Number: AB-83872
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthetic peptide of human DNA-PKCS
Reactivity: Hu, Ms
Applications:

Western Blot: 1:500 -1:2000
Immunohistochemistry: 1:50 – 1:200
Immunofluorescence: 1:50 – 1:200

Molecular: 469kDa
Purification: Aff. Pur.
Synonyms: DNA-PKCS; DNA-PKcs; DNAPK; DNPK1; HYRC; HYRC1; IM D26; XRCC7; p350; DNA-dependent protein kinase catalytic subunit
Background:

This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNAPK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination.

Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-83872-1.jpg

Western blot analysis of extracts of various cell lines, using DNA-PKCS antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 90s.

AB-83872-2.jpg

Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using DNA-PKCS antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using DNA-PKCS antibody at dilution of 1:100 (40x lens).

AB-83872-4.jpg

Immunofluorescence analysis of U-2OS cells using DNA-PKCS antibody at dilution of 1:100. Blue: DAPI for nuclear staining.