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DRP1 Monoclonal Antibody

Cat Number: MAB-94733
Conjugate: Unconjugated
Size: 100 ug
Clone: DP10
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant protein of human DRP1
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 – 1:2000
Molecular Weight: 78KDa, 82KDa
Purification: Aff. Pur.
Synonyms: DNM1L;DLP1;DRP1;DVLP;DYMPLE;EMPF;EMPF1;HDYNIV
Background:

This gene encodes a member of the dynamin superfamily of GTPases. The encoded
protein mediates mitochondrial and peroxisomal division, and is involved in
developmentally regulated apoptosis and programmed necrosis. Dysfunction of this
gene is implicated in several neurological disorders, including Alzheimer’s disease.
Mutations in this gene are associated with the autosomal dominant disorder,
encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission
(EMPF). Alternative splicing results in multiple transcript variants encoding different
isoforms.
Form: Liquid
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles

Western blot analysis of extracts of various cell lines, using DRP1
antibody at 1:7500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 30s

Western blot analysis of extracts of NIH/3T3 cells, using DRP1
antibody at 1:7500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 180s.