EGFR / HER1 Rabbit Polyclonal Antibody
| Cat Number: | AB-81864 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR |
| Reactivity: | Human,Mouse,Rat |
| Applications: | Western Blot: 1:1000 – 1:5000 |
| Molecular Weight: | 175kDa |
| Purification: | Aff. Pur. |
| Synonyms: | ERBB; ERRP; HER1; mENA; ERBB1; PIG61; NISBD2; EGFR |
| Background: | The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. |
| Form: | Liquid |
| Buffer: | PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | Store at -20°C, and avoid repeat freeze-thaw cycles. |
Western blot analysis of lysates from A431 cells, using EGFR Rabbit pAb at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 3s.
Immunohistochemistry analysis of paraffinembedded Rat liver using EGFR Rabbit pAb at dilution of 1:200 (40xlens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffinembedded Human placenta using EGFR Rabbit pAb at dilution of 1:200
(40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining
protocol.
Immunohistochemistry analysis of paraffinembedded Mouse heart using EGFR Rabbit pAb at dilution of 1:200 (40x
lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of A-431 cells using EGFR Rabbit pAb at dilution of 1:300 (40x lens).
Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg EGFR antibody. Western blot was performed from the immunoprecipitate using EGFR antibody at a dilution of 1:1000.