EGFR / HER1
| Cat Number: | AB-84336 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Synthesized peptide derived from human EGFR around the non-phosphorylation site of Y1016. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500-1:2000 |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen. |
| Background: | EGFR polyclonal antibody detects endogenous levels of EGFR protein. |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20°C, and avoid repeat freeze-thaw cycles. |
Western Blot analysis of various cell using EGFR antibody at dilution of 1:2000
Immunohistochemical analysis of paraffin-embedded Human- Appendix tissue. 1, EGFR Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-lung
tissue. 1, EGFR Polyclonal Antibody was diluted at 1:200 (4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibodyretrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of mouse-liver tissue.
1, EGFR Polyclonal antibody (red) was diluted at 1:200 (+4°C), overnight). 2, Cy3 labeled Secondary antibody was diluted at 1:300 (room temperature, 50min) 3, Picture B: DAPI (Blue) 10 min.
Picture A: Target
Picture B: DAPI
Picture C: merge A+B