EIF2A
Cat Number: | AB-84175 |
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Size: | 100 ug |
Clone: | POLY |
Concentration: | 1mg/ml |
Host: | Rb |
Isotype: | igG |
Immunogen: | A synthetic peptide of human EIF2A |
Reactivity: | Hu, Ms |
Applications: | Western Blot: 1:1000 |
Molecular Weight: | 65kDa |
Purification: | Aff. Pur. |
Synonyms: | EIF2A; CDA02; EIF-2A; MST089; MSTP004; MSTP089; eukaryotic translation initiation factor 2A. |
Background: | This gene encodes a eukaryotic translation initiation factor that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)- directed synthesis of polyphenylalanine at low concentrations of Mg2+. This gene should not be confused with eIF2-alpha (EIF2S1, Gene ID: 1965), the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40 S ribosomal subunit, the encoded protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. |
Form: | Liquid |
Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3 |
Storage: | Store at -20°C, and avoid repeat freeze-thaw cycles. |
Western blot analysis of extracts of
U-87MG cells, using EIF2A antibody
at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.