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EPO Rabbit Polyclonal Antibody

Cat Number: AB-81525
Conjugate: Unconjugated
Size: 100μg
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-193 of human EPO
Reactivity: Human, Mouse, Rat
Applications:

Western Blot: 1:500 – 1:1000
Immunohistochemistry: 1:100 – 1:200
Immunofluorescence: 1:50 – 1:200
Immunocytochemistry: 1:50 – 1:200
Molecular Weight: 21kDa
Purification: Aff. Pur.
Synonyms: EPO;EP;MVCD2
Background:

This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.
Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-81525-1-154x300

Western blot analysis of extracts of various cell lines, using EPO antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 60s.

AB-81525-2

Immunohistochemistry of paraffinembedded human esophagus using EPO
antibody (40x lens).Perform microwave antigen retrieval with 10 mM
PBS buffer pH 7.2 before commencing with IHC staining protocol.

AB-81525-3

Immunohistochemistry of paraffinembedded rat liver using EPO antibody
(40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH
7.2 before commencing with IHC staining protocol.

AB-81525-4

Immunohistochemistry of paraffinembedded human liver damage using EPO antibody (40x lens).
Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with
IHC staining protocol.

AB-81525-5

Immunofluorescence analysis of mouse
liver cells using EPO Rabbit pAb at
dilution of 1:200 (40x lens).
Blue: DAPI for nuclear staining.

References:

References for EPO Rabbit pAb
Product:EPO Rabbit pAb
Journal:Journal of Cardiovascular pharmacology
Application:WB
IF:3.105
Species:Rattus norvegicus
PMID:29438215
Title:Rutaecarpine Suppresses Proliferation and Promotes Apoptosis of Human Pulmonary ArterySmooth Muscle Cells in Hypoxia Possibly Through HIF-1α-Dependent Pathways.

References:

References for EPO Rabbit pAb
Product:EPO Rabbit pAb
Journal:Toxicol Lett
Application:WB
IF:4.372
Species:Rattus norvegicus
PMID:32461003
Title:Differential susceptibility of PC12 and BRL cells and the regulatory role of HIF-1α signaling pathway in response to acute methylmercury exposure under normoxia