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Fibronectin

Cat Number: MAB-10538
Size: 100 ug
Clone: BLAb52
Concentration: 1mg/ml
Host: Ms
Isotype: IgG1
Reactivity: Hu
Applications:

Elisa: 2-10 ug/ml
Immunohistochemistry: 2-10 ug/ml
Immunofluorescence: 2-10 ug/ml
Western Blot: 1:1000
FACS: 2-10 ug/ml
Immunofluorescence: 1:100

Purification: Purified
Background:

Fibronectin is a multifunctional glycoprotein present in connective tissues and plasma. Fibronectin mediates cell adhesion and migration during embryonic development, wound healing in adult tissues. A soluble form of fibronectin is present in blood plasma and other body fluids such as synovial and tear fluids. Plasma fibronectin is a potent opsonizing factor promoting phagocytosis of tissues and collagen debris by macrophages.

Form: Liquid
Buffer: The antibody is purified on ProteinA-Sepharose and it is desolved in PBS with 0.05% sodium azide as preservative. At this concentration azide will be toxic to most cellular systems. If necessary it can be removed through dialysis against an appropriate buffer prior to use.
Storage: The antibody can be stored frozen at -20°C.

References

Tarone G, Amedeo MR, Di Renzo MF, Comoglio P. Monoclonal antibodies to the collagen binding domain of human plasma fibronectin. Exp Cell Biol. 1984;52(4):225-36.
-Ruoslahti E. Fibronectin and its integrin receptors in cancer. Adv Cancer Res. 1999;76:1-20.
-Armstrong PB, Armstrong MT. Intercellular invasion and the organizational stability of tissues: a role for fibronectin. Biochim Biophys Acta. 2000 Mar 27;1470(2):O9-20.
-Barilla ML, Carsons SE. Fibronectin fragments and their role in inflammatory arthritis. Semin Arthritis Rheum. 2000 Feb;29(4):252-65.
-Andersen HF. Use of fetal fibronectin in women at risk for preterm delivery. Clin Obstet Gynecol. 2000 Dec;43(4):746-58. AK1540