Flag Tag
| Cat Number: | MAB-94573 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | 1E6 |
| Host: | Ms |
| Isotype: | IgG1 |
| Immunogen: | Synthetic Peptide |
| Reactivity: | All Species |
| Applications: | Western Blot: 1:5000 |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity chromatography using specific immunogen. |
| Background: | The antibody detects C-terminal, internal, and N-terminal Flag-tag fusion proteins. |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20°C, and avoid repeat freeze-thaw cycles. |
Western Blot (WB) analysis
of Recombinant protein,
diluted at 1) 1:2,500,
2) 1:5000
1ug Flag fusion protein+ Primary antibody dilution at 1:5000
IP antibody use: 5ug Flag Mouse IgG1 per ml Lysate, WB 1:5000
1) Untransfected 293 cell lysate
2) transfected 293 cell lysate with Flag-tag fusion protein
3) IP (transfected 293+ normal Mouse IgG+Protein G agarose)
4) IP (transfected 293+anti- Flag mA)
Immunofluorescence (IF) analysis of 293 cells transfected with a Flag-tag protein,1:2000 dilution (blue DAPI, red anti-Flag)