FOXO1A
| Cat Number: | AB-82717 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide of human FOXO1 |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:1000 |
| Molecular Weight: | 85kDa |
| Purification: | Aff. Pur. |
| Synonyms: | FOXO1; FKH1; FKHR; FOXO1A; forkhead box O1 |
| Background: | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C, and avoid repeat freeze-thaw cycles. |
Western blot analysis of extracts of
various cell lines, using FOXO1
antibody at 1:950 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST
Western blot analysis of extracts of
various cell lines, using FOXO1
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus
Exposure time: 90s.
Immunofluorescence analysis of HeLa
cells using FOXO1 antibody
Blue: DAPI for nuclear staining.
Application: Western blot (WB)
Sample 293T
Loading Amount 20 ug
Dilution1:1000
Incubatition timeover night
Temperature 4 ℃
Exposure 15s
Observed Band 70 kDa
Remarks Western blot analysis of extracts from HEK 293 cells and DLD-1 cells using FOXO1 Antibody (1:1000)
Application Immunofluorescence(IF)
Sample SW480
Dilution 1:200
Incubatition time over night
Temperature 4 ℃
Remarks Immunofluoresent images of SW480 cells showing nuclear and cytoplasmic localization with FOXO1 Antibody 1:200 (left)
Application: Western blot (WB)
Sample HEK293T,DLD-1
Loading Amount25 ug
Dilution 1:1000
Incubatition time over night
Temperature 4 ℃
Exposure 5s
Observed Band 70 kDa
Remarks Western blot analysis of extracts of various cell lines, using FOXO1 Polyclonal Antibody.