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FOXO1A

Cat Number: AB-82717
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthetic peptide of human FOXO1
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 – 1:1000
Immunofluorescence: 1:50 – 1:100

Molecular: 85kDa
Purification: Aff. Pur.
Synonyms: FOXO1; FKH1; FKHR; FOXO1A; forkhead box O1
Background:

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.

Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20°C, and avoid repeat freeze-thaw cycles.
AB-82717-1.jpg

Western blot analysis of extracts of
various cell lines, using FOXO1
antibody at 1:950 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST

AB-82717-2.jpg

Western blot analysis of extracts of
various cell lines, using FOXO1
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus
Exposure time: 90s.

AB-82717-3.jpg

Immunofluorescence analysis of HeLa
cells using FOXO1 antibody
Blue: DAPI for nuclear staining.

AB-82717-4.jpg

Application: Western blot (WB)
Sample 293T
Loading Amount 20 ug
Dilution1:1000
Incubatition timeover night
Temperature 4 ℃
Exposure 15s
Observed Band 70 kDa
Remarks Western blot analysis of extracts from HEK 293 cells and DLD-1 cells using FOXO1 Antibody (1:1000)

AB-82717-5.jpg

Application Immunofluorescence(IF)
Sample SW480
Dilution 1:200
Incubatition time over night
Temperature 4 ℃
Remarks Immunofluoresent images of SW480 cells showing nuclear and cytoplasmic localization with FOXO1 Antibody 1:200 (left)

AB-82717-6.jpg

Application: Western blot (WB)
Sample HEK293T,DLD-1
Loading Amount25 ug
Dilution 1:1000
Incubatition time over night
Temperature 4 ℃
Exposure 5s
Observed Band 70 kDa
Remarks Western blot analysis of extracts of various cell lines, using FOXO1 Polyclonal Antibody.