GABA A receptor alpha-2
| Cat Number: | AB-83890 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ul |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 300-400 of human GABRA2. |
| Reactivity: | Hu, Ms, |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 50kDa |
| Purification: | Purified |
| Synonyms: | GABRA2 |
| Background: | GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Alternatively, spliced transcript variants encoding different isoforms have been found for this gene. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20°C, and avoid repeat freeze-thaw cycles. |
Western blot analysis of extracts of
various cell lines, using GABRA2
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus.
Exposure time: 3s.
Review for GABRA2 Rabbit Polyclonal antibody.
Application: Western blot(WB)
Sample: mouse brain
Loading Amount: 15 ug
Dilution: 1:1000
Incubation time: over night
Temperature: 4 ℃
Exposure: 30s
Observed Band: 57 kDa
Remarks: Western blot analysis of extracts of mouse brain, using antibody at 1:1000 dilution.