GABA A receptor alpha-2
|Immunogen:||A synthetic peptide corresponding to a sequence within amino acids 300-400 of human GABRA2.|
Western Blot: 1:500 – 1:2000
GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Alternatively, spliced transcript variants encoding different isoforms have been found for this gene.
|Buffer:||PBS with 0.02% sodium azide,50% glycerol,pH7.3.|
|Storage:||Store at -20°C, and avoid repeat freeze-thaw cycles.|
Western blot analysis of extracts of
various cell lines, using GABRA2
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
Detection: ECL West Pico Plus.
Exposure time: 3s.
Review for GABRA2 Rabbit Polyclonal antibody.
Application: Western blot(WB)
Sample: mouse brain
Loading Amount: 15 ug
Incubation time: over night
Temperature: 4 ℃
Observed Band: 57 kDa
Remarks: Western blot analysis of extracts of mouse brain, using antibody at 1:1000 dilution.